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UID:20250804T142624EDT-94955uVvTo@132.216.98.100
DTSTAMP:20250804T182624Z
DESCRIPTION: \n\nMeasuring endosomal pH beyond the diffraction limit\n\nDou
 glas Richardson\n\nHarvard Center for Biological Imaging\n\n \n\nFluoresce
 nce-based biosensors have become essential tools for modern biology\, allo
 wing real-time monitoring of biological processes within living cells. One
  widely used application of biosensors is to monitor the acidification of 
 vesicles during endocytosis\, an essential function that aids in intracell
 ular cargo sorting and degradation. Prior to the development of super-reso
 lution fluorescence microscopy (nanoscopy)\, investigation of endosomal dy
 namics in live cells remained difficult as these structures lie at or belo
 w the ~250 nm diffraction limit of light microscopy. Therefore\, to aid in
  investigations of pH dynamics during endocytosis at the nanoscale\, we ha
 ve specifically designed a family of ratiometric endosomal pH probes for u
 se in live-cell STED nanoscopy.\n
DTSTART:20180222T170000Z
DTEND:20180222T180000Z
LOCATION:908\, McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3
 655 promenade Sir William Osler
SUMMARY:QLS Featured Seminar Series - Dr. Douglas Richardson
URL:/qls/channels/event/qls-featured-seminar-series-dr
 -douglas-richardson-285264
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